pCMX/cyclinB1-GFP (27) BioVector NTCC质粒载体菌种细胞基因保藏中心

pCMX/cyclinB1-GFP (27) BioVector NTCC质粒载体菌种细胞基因保藏中心
The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX.

The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) ‘An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
Vector backbone pCMX
(Search Vector Database)
Backbone size w/o insert (bp) 4500
Vector type Mammalian Expression
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name Cyclin B1
Species H. sapiens (human)
Entrez Gene CCNB1 (a.k.a. CCNB)
Tag / Fusion Protein
GFP (C terminal on insert)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site HindIII (unknown if destroyed)
3′ cloning site BamHI (unknown if destroyed)
5′ sequencing primer CMV-F
3′ sequencing primer GFP-R
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
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【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net