YB2/0 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心

YB2/0 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Rattus norvegicus, rat
Tissue spleen
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Derivation This line was produced by fusing Y3/Ag1.2.3 cells with spleen cells from an AO strain rat.
Comments Tested and found negative for ectromelia virus (mousepox).
The cells do not secrete either heavy or light immunoglobulin chains.
Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/mL and maintain at or below 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation Complete growth medium 95%; DMSO 5% (v/v).
References Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596
. . Methods Enzymol. 73B: 1-46, 1981.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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【Supplier来源】BioVector NTCC Inc.
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