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NS0 Cells小鼠骨髓瘤悬浮表达细胞株NSO BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99735
  • 货  号:NS0 Cells小鼠骨髓瘤悬浮表达细胞株NSO
  • 产  地:北京
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NS0 Cells小鼠骨髓瘤悬浮表达细胞株NSO BioVector NTCC质粒载体菌种细胞基因保藏中心
NS0 Cells小鼠骨髓瘤悬浮表达细胞株
Cell Line Name: NS0
Keywords: Mouse myeloma
Cell Line Description: NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1
(P3/NS1/1-Ag4-1). The cells are resistant to 10μ M azaguanine, cells die in the presence
of HAT medium.
Species: Mouse
Tissue: myeloma
Morphology: Lymphoblast
Growth Mode: Suspension
Subculture Routine: Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells can adhere
loosely to the culture vessel surface; remove by tapping against the flask. Cultures that
are more than 2 days old may have to be scraped.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: Not specified
Originator: Yes
Country: UK
References: Methods in Enzymology 1981;73B:3
Culture Medium: NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM glutamine. When freezing cells
down use NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM glutamine + 10% DMSO.
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