NS0 Cells小鼠骨髓瘤悬浮表达细胞株NSO BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥99735
- 货 号:NS0 Cells小鼠骨髓瘤悬浮表达细胞株NSO
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- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
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手机:18901268599
地址:北京
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NS0 Cells小鼠骨髓瘤悬浮表达细胞株NSO BioVector NTCC质粒载体菌种细胞基因保藏中心
NS0 Cells小鼠骨髓瘤悬浮表达细胞株
Cell Line Name: NS0
Keywords: Mouse myeloma
Cell Line Description: NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1
(P3/NS1/1-Ag4-1). The cells are resistant to 10μ M azaguanine, cells die in the presence
of HAT medium.
Species: Mouse
Tissue: myeloma
Morphology: Lymphoblast
Growth Mode: Suspension
Subculture Routine: Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells can adhere
loosely to the culture vessel surface; remove by tapping against the flask. Cultures that
are more than 2 days old may have to be scraped.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: Not specified
Originator: Yes
Country: UK
References: Methods in Enzymology 1981;73B:3
Culture Medium: NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM glutamine. When freezing cells
down use NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM glutamine + 10% DMSO.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
NS0 Cells小鼠骨髓瘤悬浮表达细胞株
Cell Line Name: NS0
Keywords: Mouse myeloma
Cell Line Description: NS0 is a non-Ig secreting, non-light chain-synthesising subclone of NS-1
(P3/NS1/1-Ag4-1). The cells are resistant to 10μ M azaguanine, cells die in the presence
of HAT medium.
Species: Mouse
Tissue: myeloma
Morphology: Lymphoblast
Growth Mode: Suspension
Subculture Routine: Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells can adhere
loosely to the culture vessel surface; remove by tapping against the flask. Cultures that
are more than 2 days old may have to be scraped.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: Not specified
Originator: Yes
Country: UK
References: Methods in Enzymology 1981;73B:3
Culture Medium: NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM glutamine. When freezing cells
down use NS0 Serum-Free Medium for NS0 Cells (Sigma 14650C) + 8mM glutamine + 10% DMSO.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
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