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SK-MEL-28 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥19862
  • 货  号:SK-MEL-28 cell line细胞株
  • 产  地:北京
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SK-MEL-28 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
SK-MEL-28 cell line细胞株
Organism Homo sapiens, human
Tissue skin
Cell Type Melanoma
Product Format frozen
Morphology polygonal
Culture Properties adherent
Biosafety Level 1
Disease malignant melanoma
Age 51 years adult
Gender male
Applications This cell line is a suitable transfection host.
Karyotype modal number = 90; range = 81 to 96. This is a hypotetraploid human cell line with the modal chromosome number of 90, occurring in 50% of cells. The rate of cells with a higher ploidy was 3.6%. This cell line has a very complex karyotype. There were 18 or more marker chromosomes that were common to most cells. Markers der(1)t(1;2) (p36.1;q21), del(1) (q2101) and several others had two copies per cell and t(2p14q), t(3q7p) and others had a single copy per cell. The Y/autosome translocation marker was identified as der(20)t(Y;20) (q11.23;q13.3) and had two copies per cell. The inclusion of a short segment of the euchromatic Yq11.23 was confirmed by the Southern blot DNA analysis. There were two normal X chromosomes per cell; Normal Y, N1 and N11 were absent; N19 had five or more copies per cell.
Derivation
Clinical Data male
Antigen Expression Antigen expression: Blood Type A; Rh+; HLA A11, A26, B40, DRw4
Tumorigenic Yes
Effects Yes, in nude mice; forms malignant melanoma (large round cell type)
Comments This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates.
Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing 1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,12
D5S818: 11,13
D7S820: 10,9.3
THO1: 7
TPOX: 8,12
vWA: 16,19
Isoenzymes AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1
Deposited As Homo sapiens
References Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034

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