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荧光活体成像质粒推荐-远红外荧光质粒,近红外荧光质粒,光激活荧光质粒

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Fluorescent Protein Guide: In Vivo Imaging荧光活体成像质粒

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Background

In_Vivo_Imaging.jpg

In vivo imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent probes are suitable for stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have emission near or above 650 nm as signals below 650 nm are highly absorbed by hemoglobin and cause undesirable background signals.

Near-infrared fluorescent proteins for in vivo imaging are derived from bacterial phytochrome photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging. Photo-activatable iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially selective imaging of tissues in living animals. Further development of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein interactions in vitro and in vivo.

Learn more about in vivo imaging tools in this blog post - In Living Color: The Skinny on In Vivo Imaging Tools.

The following far red, near-infrared, and photoactivatable fluorescent empty backbone plasmids are ideal for single or multicolor in vivo imaging.

Plasmids for In Vivo Imaging

Far Red远红外荧光质粒

ProteinExcitation/EmissionBrightnessFind Plasmids
mCardinal604/65918All mCardinal plasmids from article
mNeptune600/65013.4pcDNA3-mNeptune-N
E2-Crimson611/64628.98pEF.myc.ER-E2-Crimson

Near-Infrared近红外荧光质粒

ProteinExcitation/EmissionBrightnessFind Plasmids
iRFP670643/67012.7piRFP670-N1
miRFP670642/67012pCMV-iRFP670-N, pmiRFP670-N1
iRFP682663/68210.2piRFP682-N1
iRFP702673/7027.6piRFP702-N1
miRFP703673/7038pmiRFP703-N1
miRFP709683/7094pmiRFP709-N1
iRFP (aka iRFP713)690/7136.2piRFP
iRFP720702/7205.8piRFP720-N1
iSplit690/7135.3pPAS-E and pK-GAFm

Photoactivatable光激活荧光质粒

ProteinExcitation/EmissionBrightnessFind Plasmids
PAiRFP1690/717 (after photoactivation)3.2pPAiRFP1-N1
PAiRFP2692/719 (after photoactivation)3.0pPAiRFP2-N1


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