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pDRIVE-hbAct

  • 价  格:¥9852
  • 货  号:pDRIVE-hbAct
  • 产  地:北京
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pDRIVE-hbAct
pDRIVE is an expression plasmid containing a native or composite promoter
of interest. pDRIVE may be used to:
- Subclone a promoter of interest into another vector. Unique restriction
sites are present at each end of the promoter allowing convenient excision. The
5’ sites are Sda I, and Spe I. Sda I is compatible with Nsi I and Pst I. Spe I is
compatible with Avr II, Nhe I and Xba I. The 3’ restriction site is Nco I which
includes the ATG start codon, and is compatible with BspH I and BspLU11 I.
- Compare the activity of different promotersin transient transfection experiments.
Each pDRIVE promoter drives the expression of the SEAP reporter gene which
allows for testing of the promoter’s activity in transient transfection experiments.
Furthermore, the SEAP gene is flanked by unique restriction sites (Nco I and
Nhe I) for easy replacement with a different gene of interest.
PROMOTER CHARACTERISTICS
Human beta actin promoter
Complete Promoter size: 2387 bp
Beta-actin is a highly conserved protein ubiquitously expressed in all
eukaryotic cells as a component of the cytoskeleton and as a mediator of
internal cell motility. A 1.2-kb fragment of the β-actin 5’ flanking region is
sufficient for efficient transcription1
. This 1.2-kb fragment contains a TATA
box, a CCAAT box and two highly conserved elements. The region between
+765 and +783 downstream from the cap site has enhancer activity and acts as
a serum-response element in quiescent cells2
. This 19-bp element contains a
CC(A/T)6GG sequence which is found in the promoter region of many
cytoskeletal and muscle-specific actin genes. The human β-actin promoter was
shown to drive high levels of expression of a transgene in vitro3
. InvivoGen
provides a larger fragment that includes the 5’UTR of the β-actin gene.

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