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大肠杆菌E.coli ORN178 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:大肠杆菌E.coli ORN178
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大肠杆菌E.coli ORN178

BioVector NTCC质粒载体菌种细胞基因保藏中心

The estimated risk of prostate cancer is 21 % and thelifetime risk of death is 2–5 %. Even though it can be diagnosed early andtherapy can be started immediately, patients developing metastatic conditionsdie. Apart from the potent issue of cancer, recurrent urinary tract infection(UTI) is one of the most prevalent symptoms. Escherichiacoli ORN178 are the mostcommon cause of UTI in humans. Various urovirulence factors of E. coli ORN178 have been identified such asmolecular biology of surface receptors of the urothelial cells of the urinarytract, the adhesin specificity and primarily and the type 1 fimbriae associatedwith the organism. Detection, diagnosis, and treatment of UTI play an importantrole in prostate cancer. One of the novel tools currently vastly studied arenanoparticles. Nanoparticles continue to be used as carriers for localized drugdiffusion to treat and detect infections and diseases like cancer. Owing totheir nano size, it is easy for these particles to diffuse intothe cells and effect desired responses in treatment of diseases. However, thesize, type, and surface charges of the particles play a vital role. Of thedifferent kind of nanoparticles, gold nanoparticles (GNPs) have beenextensively studied in this regard. It has been shown that E. coli ORN178 binds specifically to d-mannose, which is an integral part of theglycoproteins that are a part of the adhesive domain on host cells. Thefimbriae of the uropathogenic E. coli ORN178 bind to the urolapkins on thesurface of urothelial cells of the human bladder. d-mannose functionalized 200 nm GNPsbind specifically to E. coli ORN178. It has been shown that E. coli ORN178 binds specifically to d-mannose only and E. coli ORN208 serves as a negative controlas it has type 1 pili that fail to bind to d-mannose.


Escherichia coli is one the most commonbacteria responsible of uropathogenic diseases, which motives the search forrapid and easy methods of detection. ORN178 is a positive phenotype for thetype 1 fimbriae expression, and the ORN208 that does not express fimbriae 1 asnegative control.


Uropathogenic ORN178 genotype:

thr-1 leuB thi-1 (argF-lac)U169 xyl-7ara-13 mtl-2 gal-6 rpsL tonA2 supE44 pilG1, tetR inserted ca. 200 bp 3' to theend of fimH, Tetr λr Pil+  (does notexhibit phase variation of piliation)


The E. coli strain ORN 178 presents theideal candidate to investigate this glycan cantilever array sensor as itcontains type-I pili that specifically bind to mannose-containing structuresvia the binding protein FimH.

The use of αα-D-mannose receptors ascapturing agents for the detection of Escherichia coli using a microcantileverwas developed. Researchers have demonstrated that E. coli ORN178, whichexpresses normal type-1 pili, can interact with microcantilevers functionalizedwith αα-D-mannose and can cause shifts in its resonance frequencies. AlthoughE. coli ORN208, which expresses abnormal pili, binds poorly to αα-D-mannose onthe nitrocellulose membrane of a FAST slide, it did cause a detectable shift inresonance frequency when interacting with the αα-D-mannose functionalizedmicrocantilevers.



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