pPTluc BioVector NTCC质粒载体菌种细胞基因保藏中心

pPTluc BioVector NTCC质粒载体菌种细胞基因保藏中心
Luciferase reporter for testing enhancer fragments in Drosophila
Vector backbone pPTGAL
(Search Vector Database)
Backbone manufacturer Daniel F. Eberl lab, PMID 12324963
Backbone size (bp) 9890
Modifications to backbone replace a 3193bp fragment containing Gal4 flanked by PstI sites with a 1955bp HindIII-BamHI fragment containing the luciferase gene from pGL3-Basic (Promega).
Vector type Insect Expression
Promoter minimal promoter
Tag / Fusion Protein
luciferase (C terminal on backbone)
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Unknown
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ sequencing primer pCasper-F
3′ sequencing primer lucNRev
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net