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Pax7-pSAM2 BioVector NTCC质粒载体菌种细胞基因保藏中心
The PSAM2-PAX7 vector contained a tet-responsive
element to induce Pax7 expression by doxycycline (Dox)
and an internal ribosome entry site (IRES)-EGFP reporter to assess
transgene expression through green fluorescence. Inducible
expression of Pax7 was confirmed based on EGFP (Pax7) expression,
following an overnight incubation of ES/iPS cells with 0.75 μg/
mL dox. Cells were then harvested and differentiated as embryoid
bodies (EBs).
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
The PSAM2-PAX7 vector contained a tet-responsive
element to induce Pax7 expression by doxycycline (Dox)
and an internal ribosome entry site (IRES)-EGFP reporter to assess
transgene expression through green fluorescence. Inducible
expression of Pax7 was confirmed based on EGFP (Pax7) expression,
following an overnight incubation of ES/iPS cells with 0.75 μg/
mL dox. Cells were then harvested and differentiated as embryoid
bodies (EBs).
【Supplier来源】BioVector NTCC Inc.
TEL:+86-010-53513060
【Website网址】 http://www.biovector.net
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