pLVTH plasmid慢病毒RNAi载体shRNA表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pLVTH plasmid慢病毒RNAi载体shRNA表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pLVTH plasmid慢病毒RNAi载体shRNA表达质粒
This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA.

For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid).

Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning.
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