LRG (Lenti_sgRNA_EFS_GFP)慢病毒基因编辑sgRNA表达荧光报告质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

LRG (Lenti_sgRNA_EFS_GFP)慢病毒基因编辑sgRNA表达荧光报告质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
LRG (Lenti_sgRNA_EFS_GFP)慢病毒基因编辑sgRNA表达荧光报告质粒，在哺乳动物细胞中组成型表达sgRNA连接GFP报告基因，慢病毒载体骨架。
(Empty Backbone) Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.
Vector backbone Lenti virus
(Search Vector Database)
Backbone manufacturer Modified from lentiCRISPRv1 (Plasmid 49535)
Backbone size (bp) 9294
Vector type Mammalian Expression, Lentiviral, CRISPR
Promoter U6 promoter-driven sgRNA expression and EFS promoter-driven GFP expression
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number High Copy
CLONING INFORMATION
Cloning method Ligation Independent Cloning
5′ sequencing primer AACCGGTGCCTAGAGAAGGT
(Common Sequencing Primers)
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net