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HT29-MTX-E12 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99625
  • 货  号:HT29-MTX-E12 cell line细胞株
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HT29-MTX-E12 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: HT29-MTX-E12
Keywords: HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption
Cell Line Description: HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer. HT29-MTX-E12 provides a model system to study the influence of the mucous layer on nanoparticle diffusion.
Species: Human
Tissue: Colon
Morphology: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17
Subculture Routine: Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm².
Culture Medium: DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).
Depositor: Professor David Brayden, Associate Professor of Drug Delivery and Kevin McMahon, Senior Technical Officer, University College Dublin, UCD School of Veterinary Medicine, Belfield, Dublin 4, IRELAND. Originator: Professor Per Artursson, Uppsala University, Sweden.
Originator: No
Country: Ireland
References: Behrens I, Stenberg P, Artursson P, Kissel T. 2001 Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells. Pharm Res. 18:1138-45. PMID: 11587485.
Additional Bibliography: Keely S, Rullay A, Wilson C, Carmichael A, Carrington S, Corfield A, Haddleton DM, Brayden DJ. 2005 In vitro and ex vivo intestinal tissue models to measure mucoadhesion of poly (methacrylate) and N-trimethylated chitosan polymers. Pharm Res. 22:38-49. PMID: 15771228.

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