M-07e Cell line细胞株
BioVector NTCC质粒载体菌种细胞基因保藏中心

M-07e Cell line细胞株
BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
M-07e
Species:
human (Homo sapiens)
Cell type:
acute megakaryoblastic leukemia
Origin:
established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
single round cells in suspension, a few cells may be spindle-like and slightly adherent;
image

Medium:
80-90% Iscove's MDM or RPMI 1640 + 10-20% h.i. FBS + IL-3 (10 ng/ml) or GM-CSF (10 ng/ml) or 10-20% vol conditioned medium of cell line 5637
Subculture:
seed out at about 0.5 x 106 cells/ml and maintain at 0.5-1.0 x 106cells/ml; split 1:2 to 1:3 every two days; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40 hours
Harvest:
cell harvest at best at ca. 1 x 106 cells/ml; maximal density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human near-diploid karyotype - 46(45-46)<2n>XX, t(11;21)(p11;p13), add(13)(p13), add(22)(p13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

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