SW1222 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

SW1222 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Description: The SW1222 cell line was established from a patient with a moderately well differentiated adenocarcinoma of the colon classified as Dukes' stage C. SW1222 cells produce tumours in female athymic nu/nu nude mice.



Also Known As:



Species: Human



Tissue: Colon



Growth Properties: Adherent



Morphology: Epithelial



Growth Medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine



Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.



Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.



Depositor: Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD. Originator: Dr A Leibovitz deposited by agreement with Dr Meenhard Herlyn Director of the Wistar Institute, 3601 Spruce St Philadelphia PA 19104



Originator: Yes



References: Richman PI, Bodmer WF. 1988 Control of differentiation in human colorectal carcinoma cell lines: epithelial-mesenchymal interactions. J Pathol.156(3):197-211 PMID: 3204451. Browning MJ, Krausa P, Rowan A, Hill AB, Bicknell DC, Bodmer JG, Bodmer WF.1993 Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. J Immunother Emphasis Tumor Immunol. 14(3):163-8. PMID: 8297898.
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