pCMV-Rev BioVector NTCC质粒载体菌种细胞基因保藏中心

pCMV-Rev BioVector NTCC质粒载体菌种细胞基因保藏中心
Description:
pCMV-Rev表达质粒，哺乳动物细胞中表达rev蛋白，位于CMV启动子和 rabbit ß-globin splice和poly A信号之间。

A 722 bp fragment from pCV-1 containing HIV-1 rev cDNA was obtained by Bsu36I digestion. This fragment was blunt end ligated into the BamHI site of pCMV to generate the 4972 bp plasmid pCMV-rev.

Special Characteristics:

This construct is 4944 bp.

Expresses rev protein upon transfection into mammalian cells. Expression is driven by the simian CMV immediate early promoter. The rev cDNA is identical to the rev sequences cloned into plasmid pSV-rev (Catalog #1444), and is positioned between the CMV promoter and the rabbit ß-globin splice and poly A signals.
Source of Pro Virus: pCV-1 (cDNA clone, Catalog #303).

Plasmid map and sequence file lot 130071

This reagent is currently being provided as dried purified DNA stabilized in DNAstable PLUS. Please see the notice for additional information and the protocol for reconstitution of dried DNA reagents. Dried DNA Notice

Cloning Site:

BamHI

Cloning Vector:

pCMV

Ampicillin resistance

Contributor:

Dr. Marie-Louise Hammarskjöld and Dr. David Rekosh.

References:

Lewis N, Williams J, Rekosh D, Hammarskjöld M-L. Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV-2) that is responsive to the HIV-1 rev and human T-cell leukemia virus types I and II rex proteins. J Virol 64:1690-1697, 1990.
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