Fusarium graminearum BioVector NTCC质粒载体菌种细胞基因保藏中心

Fusarium graminearum BioVector NTCC质粒载体菌种细胞基因保藏中心
Strain Designation: NRRL 5883
Deposited Name: Fusarium graminearum Schwabe
Product Description: An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia)
suspended in cryoprotectant.
The information recommended in this section is to assist users in obtaining living culture(s) for their studies.
The recommendation does not imply that the conditions or procedures provided below are optimum.
Experienced researchers may initiate the growth of a culture in their own way.
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Recommended Procedure
For freezedry
(lyophilized) ampoules:
1. Open an ampoule according to enclosed instructions.
2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL
with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g.,
overnight) rehydration might increase viability of some fungi.
5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended
solid or liquid medium. Include a control that receives no inoculum.
6. Incubate the inoculum at the propagation conditions recommended.
7. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 23
days of incubation. However, the time necessary for significant growth will vary from strain to strain.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net