Mav203酿酒酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Mav203酿酒酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
S. cerevisiae strains MaV103 (MATa) andMaV203 (MATα) contain deletions of the GAL4 and GAL80 genes encoding Gal4p and its repressor Gal80p, respectively. In the absence of Gal80p, galactose is not required for activation of Gal4p-inducible promoters. The strains contain three Gal4p inducible reporter genes, providing four phenotypes to identify true interactors. The reporter genes are HIS3, providing for growth on plates lacking histidine, LacZ, for colorimetric detection of Gal4p activity, and URA3, providing growth on media lacking uracil, as well as sensitivity to the drug 5-FOA. In addition, the strains are resistant to canavanine and cycloheximide, aiding in plasmid shuffling.

MaV103 and MaV203 were derived by Marc Vidal from a cross between two non-isogenic strains, PCY2 and MaV99, and are described in more detail in Chevray and Nathans 1992, Vidal et. al. 1996, and in "The Reverse Two-Hybrid system, M. Vidal" in "The yeast two-hybrid system" ed. P. L. Bartel and S. Fields.
MaV203 Competent Yeast Cells are designed for use with the ProQuest™ Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest™ bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors (Figure 2). The MaV203 competent cells feature:

• Transformation (250 µl of cells) efficiency yields >1 x 106 colonies
• Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media
• Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
• Competent cells formatted with carrier DNA already included
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