Y8930 S. cerevisiae strain酿酒酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Y8930 S. cerevisiae strain酿酒酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Y8800 and Y8930
S. cerevisiae strains Y8800 (MATa) and Y8930 (MATα) contain deletions of the GAL4 and GAL80 genes encoding Gal4p and its repressor Gal80p, respectively. In the absence of Gal80p, galactose is not required for activation of Gal4p-inducible promoters. The strains contain three Gal4p inducible reporter genes, providing four phenotypes to identify true interactors. The reporter genes are HIS3, providing for growth on plates lacking histidine, LacZ, for colorimetric detection of Gal4p activity, and ADE2, providing growth on media lacking adenine, as well as a range of color from red to white depending on the strength of Gal4p expression. In addition, the strains are resistant to cycloheximide, aiding in plasmid shuffling.

Y8800 and Y8930 were generated by X. Xin in C. Boone's laboratory by adding cycloheximide resistance to the PJ69-4 Y2H strains. PJ69-4 is described in P. James et. al. 1996.
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