DAN-G cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

DAN-G cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: DAN-G DSMZ no.: ACC 249Species: human (Homo sapiens)Cell type: pancreas carcinoma Origin: established from a human individual with pancreas carcinoma in 1985 Reference(s): not publishedBiosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:adherent epitheloid cells growing in monolayers; image; imageMedium: 90% RPMI 1640 + 10% h.i. FBS Subculture: seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA Incubation: at 37 °C with 5% CO2 Doubling time: about 33 hours Harvest: cell harvest of about 1-3 x 105 cells/cm2 Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed originally as human with IEF of AST, NP Cytogenetics: human irregular hypotriploid karyotype with 3.5% polyploidy - 59(52-61)<3n>der(X), -X/Y, -X/Y, -1, -2, -3, -4, -5, +6, +7, -9, -10, -13, -16, -17, -18, -20, -22, -22, +4mar, add(2)(p?16), add(4)(q25), i(5p), add(6)(p22), add(7)(p11/13), der(7)t(3;7)(q11;p11), del(7)(p13), add(8)(p11), add(9)(p21), add(12)(p11), i(13q), der(14)t(14;?22)(q32;q12), add(15)(q26), der(19)t(19;hsr)(p13;?), add(20)(q13) Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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