PA-TU-8988S cell line人胰腺癌细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

PA-TU-8988S cell line人胰腺癌细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: PA-TU-8988S DSMZ no.: ACC 204Species: human (Homo sapiens)Cell type: pancreas adenocarcinoma Origin: established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman (sister cell line is PA-TU-8988T, DSM ACC 162) Reference(s): 14508Biosafety level: 1 Permissions and restrictions: A  DSMZ Cell Culture Data: Morphology:adherent epitheloid cells growing in monolayers; image; imageMedium: 90% Dulbecco's MEM + 5% h.i. FBS + 5% horse serum + 2 mM L-glutamine Subculture: split confluent culture 1:10 once a week using trypsin/EDTA; seed out at ca. 4 x 106 cells/80 cm2 Incubation: at 37 °C with 10% CO2 Doubling time: about 40-60 hours Harvest: cell harvest of about 30 x 106 cells/80 cm2 Storage: frozen with 70% medium, 20% FBS, 10% DMSO  DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Fingerprint: same DNA profile as PA-TU-8988Tusing multiplex PCR of minisatellite markers Species: confirmed as human with IEF of AST, MDH Cytogenetics: human near triploid karyotype with 6% polyploidy - 71(67-77)<3n>XXX, +3, -4, +7, -8, +9, +11, +11, -15, -17, -18, -18, -19, -21, -22, +6mar, del(1)(p21), del(3)(p11p22), del(6)(q26), del(9)(p12), dup(11)(p14->pter), del(12)(p13), add (15)(p12) Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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