HBEC3-KT cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HBEC3-KT cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line name HBEC3-KT
Synonyms HBEC3 KT; HBEC 3KT; HBEC3KT; HBEC3

Organism Homo sapiens, human
Tissue lung, bronchial
Cell Type epithelial
Product Format frozen
Morphology epithelial, packed cuboidal
Culture Properties adherent
Biosafety Level 2 [Cells immortalized by CDK4 and hTERT and contain SV40 promoter sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease normal
Age 65
Gender female
Applications
HBEC3-KT cells are normal human bronchial epithelial cells immortalized with CDK4 and hTERT. The immortalized HBEC3-KT cells do not form colonies in soft agar, nor do they form tumors in mice (Ref). Multiple oncogenic changes (K-RAS(V12), p53 knockdown, mutant EGFRs) are not sufficient to confer a full malignant phenotype on HBEC3-KT. These additional genetic changes, commonly found in human lung cancer, progress the HBEC3-KT cells partially, but not completely, toward malignancy (Ref).

Extended lifespan (Ref), multi-potent differentiation capacity in three-demensional models (Ref), and drug-sensitivity tests (paclitaxel, carboplatin, pemetrexed, cisplatin, gemcitabine, etc.) have been reported for the HBEC3-KT cells

Comments Transfected with: HGNC; 11730; TERT.
Transfected with: MGI; MGI:88357; Cdk4.
Omics: miRNA expression profiling.
Omics: Transcriptome analysis.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_EQ78 (HBEC3KT53)
Sex of cell Female
Age at sampling 65Y
Category Telomerase immortalized cell line
STR profile Source(s): ATCC

Markers:
Amelogenin X
CSF1PO 10,11
D13S317 12,13
D16S539 9,12
D5S818 12
D7S820 8,12
TH01 9.3
TPOX 11,12
vWA 15,17
Web pages http://www.evercyte.com/hbec3-kt-161.html
Publications
PubMed=15604268; DOI=10.1158/0008-5472.CAN-04-3703
Ramirez R.D., Sheridan S., Girard L., Sato M., Kim Y., Pollack J., Peyton M., Zou Y., Kurie J.M., Dimaio J.M., Milchgrub S., Smith A.L., Souza R.F., Gilbey L., Zhang X., Gandia K., Vaughan M.B., Wright W.E., Gazdar A.F., Shay J.W., Minna J.D.
Immortalization of human bronchial epithelial cells in the absence of viral oncoproteins.
Cancer Res. 64:9027-9034(2004)
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net