MCF 10A cell line人乳腺上皮细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MCF 10A cell line人乳腺上皮细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
MCF 10A cell line人乳腺上皮细胞株
Organism: Homo sapiens, human / Cell Type: epithelial / Tissue: mammary gland; breast / Disease: fibrocystic disease

Organism Homo sapiens, human
Tissue mammary gland; breast
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease fibrocystic disease
Age 36 years
Gender female
Ethnicity Caucasian
Applications This line is suitable as a transfection host.
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Derivation MCF 10A was derived from adherent cells in the population.
Clinical Data 36 years
Caucasian
female
Tumorigenic No
Effects No, in immunosuppressed mice
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Yes, in semisolid medium
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Comments The MCF 10A cell line is a non-tumorigenic epithelial cell line.
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The calcium content of the medium exerts a strong effect on the morphology of the cells.
Complete Growth Medium The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. NTCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. To make the complete growth medium, you will need to add the following components to the kit (sold separately):
• 100 ng/ml cholera toxin
Note: Do not filter complete medium
Subculturing Remove medium and rinse monolayer with PBS (NTCC® 30-2200™). Add 3.0 mL 0.05% trypsin, 0.53 mM EDTA and incubate at 37°C for 15 minutes. To neutralize trypsin, add 3 mL solution of 0.1% soybean trypsin inhibitor (NTCC® 30-2014™). Centrifuge cell suspension at 125 x g for 5 to 10 minutes. Resuspend cell pellet in complete culture medium. Add appropriate aliquots of cell suspension to new culture vessels. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17

Isoenzymes
AK-1, 1
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ES-D, 1
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G6PD, B
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GLO-I, 1-2
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PGM1, 1-2
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PGM3, 1
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U.S. Patent Number 5,026,637

【Supplier来源】BioVector NTCC Inc.
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