NAMALWA cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NAMALWA cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
NAMALWA
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the tumor mass of an African child with Burkitt lymphoma in 1967; cells express MYC mRNA
Reference(s):
18146
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV on handling the cells is unlikely. Additionally, this cell line carries sequences of the squirrel monkey retrovirus (SMRV). According to a recommendation of the German Commission for Biosafety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used for genetic manipulations.

NTCC Cell Culture Data:
Morphology:
lymphoblastoid, single cells or small clusters in suspension; image;
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 20-30 hours
Harvest:
maximum density at about 2.0 x 106 cells/ml or higher
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, NP
Cytogenetics:
human hypodiploid karyotype with 4% polyploidy - 44(43-46)<2n>X, -Y, -5, -6, +7, -10, -13, -21, +3mar, ins(1;?)(p11;?), ins(3)(q11q29), der(5)t(1;5)(q25;q35), del(8)(p22), der(14)t(14;?)(p11;?), der(15)t(15;21)(p11;q11) - a supernumerary ring or fragment present in 8% of cells - NAMALWA marker chromosomes present
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV +

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