HaCaT cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HaCaT cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Origin:
In vitro spontaneously transformed keratinocytes from histologically normal skin.
Species:
Homo sapiens
Tissue:
Skin
Morphology:
Keratinocyte
Properties:
Adherent monolayer
Cytogenic data:
Patient:
Male, Caucasian, 62 years of age.
Medium:
DMEM + 10% FBS
Subculture:
Recommended 1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X,X
CSF1PO: 9,11
D13S317: 10,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 11,12
vWA: 16,17
HaCaT Cells References:
1. Nakai, K., Yoneda, K., et al. HB-EGF-induced VEGF production and eNOS activation depend on both PI3 kinase and MAP kinase in HaCaT cells. J Dermatol Sci, 55: 170-178, 2009.
2. Markopoulou, S., Kontargiris, E., Batsi, C., Tzavaras, T., Trougakos, I., Boothman, D. A., Gonos, E. S., and Kolettas, E. Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin. Febs J, 276: 3784-3799, 2009.
3. Aksoy P, et al. HPV16 infection of HaCaTs is dependent on β4 integrin,and α6 integrin processing. Virology, Jan 20;449:45-52, 2014. PMID: 24418536
4. Mohammad H, Cushman M, Seleem MN. Antibacterial Evaluation of Synthetic Thiazole Compounds In Vitro and In Vivo in a Methicillin-ResistantStaphylococcus aureus (MRSA) Skin Infection Mouse Model. PLoS One. 2015 Nov 4;10(11):e0142321.PMID: 26536129
5. Aijaz A, Faulknor R, Berthiaume F, Olabisi RM.Hydrogel Microencapsulated Insulin-Secreting Cells Increase Keratinocyte Migration, Epidermal Thickness,Collagen Fiber Density, and Wound Closure in a Diabetic Mouse Model of Wound Healing. Tissue Eng Part A. 2015 Nov;21(21-22):2723-2732. PMID: 26239745
6. Crochiere M, Kashyap T, Kalid O, Shechter S, Klebanov B, Senapedis W, Saint-Martin JR, Landesman Y. Deciphering mechanisms of drug sensitivity and resistance to Selective Inhibitor of Nuclear Export (SINE)compounds. BMC Cancer. 2015 Nov 17;15:910. PMID: 26573568

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