出品公司: | EMD Biosciences(Novagen) |
---|---|
别名: | pET42b, pet 42b, pET-42b(+) |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5929bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-GST, N-His, C-His |
载体抗性: | Kanamycin |
备注: | 含有GST融合蛋白;N端Thrombin蛋白酶切位点; 含有N端Factor Xa蛋白酶切位点 ; pET 42 a,b,c 的差异仅在多克隆位点区域。 |
产品目录号: | 70562-3 |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pet42b载体基本信息
pet42b载体质粒图谱和多克隆位点信息
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 120 | 1 | |
T7_Terminal_primer | 69 | 87 | |
S15 | 350 | 306 | |
6xHIS | 410 | 393 | |
GST (variant) | 1091 | 441 | |
T7_transl_en_RBS | 1116 | 1100 | |
lacO | 1161 | 1134 | |
T7_promoter | 1179 | 1161 | |
tet (300 - 563) | 1215 | 1478 | |
pBRrevBam_primer | 1286 | 1267 | |
lacI | 1561 | 2652 | |
ROP | 3224 | 3415 | |
pGEX_3_primer | 3431 | 3409 | |
pBR322_origin | 4449 | 3830 | |
KanR2 | 4555 | 5370 |
ORF | Start | End | |
---|---|---|---|
ORF frame 3 | 1091 | 147 | |
ORF frame 3 | 1092 | 1820 | |
ORF frame 1 | 1693 | 2652 | |
ORF frame 1 | 4555 | 5370 |
Enzyme Name | Cut |
---|---|
XhoI | 174 |
NotI | 182 |
EagI | 182 |
HindIII | 189 |
SalI | 195 |
SacI | 206 |
PstI | 214 |
AscI | 216 |
StuI | 226 |
EcoRI | 235 |
BamHI | 241 |
EcoRV | 251 |
NcoI | 254 |
AgeI | 288 |
KpnI | 295 |
BglII | 298 |
SacII | 389 |
SpeI | 420 |
MscI | 883 |
NdeI | 1090 |
XbaI | 1128 |
ApaI | 2131 |
HpaI | 2426 |
NruI | 4643 |
SmaI | 4860 |
XmaI | 4858 |
pet42b载体简介
The pET-42 series is designed for cloning and high-level expression of peptide sequencesfused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encodedsequence can be completely removed when cloning into the PshAI site (as shown below) and thencleaving the GST fusion protein with Factor Xa.
pet42b载体序列
您正在向 biovector.net 发送关于产品 pet42b大肠杆菌蛋白表达载体 的询问
- 公告/新闻