pHEX2 T494 TALENs BioVector NTCC质粒载体菌种细胞基因保藏中心

pHEX2 T494 TALENs BioVector NTCC质粒载体菌种细胞基因保藏中心
Vector backbone pHEX2
(Search Vector Database)
Backbone size w/o insert (bp) 13812
Total vector size (bp) 21391
Vector type Plant Transformation
Selectable markers Kanamycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Spectinomycin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT
Gene/Insert name AtCRU3 T494 TALENs
Alt name Arabidopsis thaliana CRUCIFERIN 3 gene Target 494 TALENs
Species Synthetic
Insert Size (bp) 7549
Promoter CaMV 35S
CLONING INFORMATION
Cloning method Gateway Cloning
5′ sequencing primer RAJ-417 (CAACCACGTCTTCAAAGCAAGTG)
3′ sequencing primer m13 Forward
pZHY013 reference:
Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179

Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.

pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13

pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net