PK-15 cell line猪肾上皮细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

PK-15 cell line猪肾上皮细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
PK-15 cell line猪肾上皮细胞株
Organism Sus scrofa, pig Tissue kidney Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Age adult Applications transfection host Derivation Genes Expressed plasminogen activator; keratin,The cells are positive for porcine circovirus (PCV) antigens.,The cells are positive for keratin by immunoperoxidase staining. Cellular Products plasminogen activator; keratin Virus Resistance poliovirus 2 Comments The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources. The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that PK-15 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. The cell line harbors an endogenous C-type retrovirus. The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining. Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Rinse the cell sheet 2 times with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Cryopreservation culture medium 95%; DMSO, 5% Culture Conditions Temperature: 37.0°C Cross References Nucleotide (GenBank) : AJ133817 Porcine endogenous retrovirus type C proviral gag, pol and env genesand LTR (class A, clone 42). Nucleotide (GenBank) : AJ133816 Porcine endogenous retrovirus type C proviral gag, pol and env genes and LTR (class B, clone 33).
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