Human poliovirus 1人脊髓灰质炎病毒I型 BioVector NTCC质粒载体菌种细胞基因保藏中心

Human poliovirus 1人脊髓灰质炎病毒I型 BioVector NTCC质粒载体菌种细胞基因保藏中心
Human poliovirus 1 Cat No.: NTCC691872 Classification Picornaviridae, Enterovirus, Enterovirus C Deposited As Poliovirus 1 Agent Human poliovirus 1 Strain LSa Applications testing Biosafety Level 2 Isolation LS-a virus is a variant or mutant isolated from the Mahoney strain. (The Mahoney strain was isolated by T. Francis in 1941 from a pool of 3 stools from asymptomatic cases of poliomyelitis in Cleveland.) Product Format freeze-dried Storage Conditions -70°C or colder Comments [The BMBL notes that all laboratory personnel working directly with the agent must have documented polio vaccination or demonstrated serologic evidence of immunity to all three poliovirus types. (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4)]. Effect on Host Yes, in vitro effects: Cytopathic effects in tissue culture CPE, rounded and sloughing cells in culture. Yes, in vivo effects: paralysis in mouse Recommended Host mouse, i. s. inoculation; LLC-MK2 cells Production Host: LLC-MK2 mouse; monkey, i. s. inoculation; monkey kidney cells Growth Conditions Duration: 2-10 days Effect on Host Yes, in vitro effects: Cytopathic effects in tissue culture CPE, rounded and sloughing cells in culture. Yes, in vivo effects: paralysis in mouse
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