pUC18T-mini-Tn7T-Gm BioVector NTCC质粒载体菌种细胞基因保藏中心

pUC18T-mini-Tn7T-Gm BioVector NTCC质粒载体菌种细胞基因保藏中心

pUC18-mini-Tn7和pUC18R6K-mini-Tn7系列Tn7转座子载体，pUC18-mini-Tn7T-Gm和pUC18-mini-Tn7T-Gm-REP，pUC18-mini-Tn7，带有R6K自杀型复制子，oriT接合转移起始位点，T0T1转录终止子来自噬菌体I和rrnB操纵子，Tn7R和Tn7L是Tn7转座子的左右端。
Suicide delivery vectors and mini-Tn7 elements. (a) pUC18-based suicide delivery plasmids containing mini-Tn7 base elements. These plasmids are mobilizable derivatives of pUC18-mini-Tn7 and pUC18R6K-mini-Tn7 (for details see Supplementary Fig. 1). bla, b-lactamase–encoding gene; ori, ColE1 origin of replication; oriR6K, R6K origin of replication; oriT, origin of conjugative transfer; T0T1, transcriptional terminators T0 and T1 from bacteriophage l and E. coli rrnB operon, respectively; Tn7L and Tn7R, left and right end of Tn7, respectively. (b) Maps of selected mini-Tn7 elements. The mini-Tn7 elements are contained on the suicide delivery vectors shown in a, except mini-Tn7 which is located on pUC18. Abbreviations: aacC1, Gm acetyl transferase–encoding gene; FRT, Flp recombinase target; MCS, multiple cloning site (for mini-Tn7 and mini-Tn7T the MCS are the same as in a; in others some sites may not be unique; for details see GenBank sequences). Maps of other transposons derived from mini-Tn7 and mini-Tn7T

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