pSecTag B plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSecTag B plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
别名: pSecTagB 质粒类型: 哺乳动物表达载体 启动子: CMV 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5170 bp (查看载体序列) 载体标签: IgK Leader Sequence, c-Myc Epitope Tag, His Tag (6x) 载体抗性: Ampicillin (氨苄青霉素) 筛选标记: 博莱霉素(Zeocin)
pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features: Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2) Cytomegalovirus (CMV) promoter for high-level constitutive expression C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody C-terminal c-myc epitope for detection with an Anti-myc Antibody Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7) The pSecTag2 vectors carry the Zeocin™ resistance gene for cost-effective selection in mammalian cells. Zeocin™ selection can also be used in E. coli.The pSecTag2/Hygro vectors have the Hygromycin B resistance gene for selection of stable mammalian cell lines.
【Supplier来源】BioVector NTCC Inc.
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