M1 mouse myeloid leukemia cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

M1 mouse myeloid leukemia cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
M1
BioVector No.:
79-331
Species:
murine (mouse) (Mus musculus)
Cell type:
myeloid leukemia
Origin:
bone marrow from SL mouse with spontaneous myeloid leukemia was injected into isogenic mice and the cell line was established from the ensuing hyperplastic lymph nodes 14 days later by recloning on soft agar; homotransplantable and forms colonies on soft agar (granulocytic-macrophage differentation inducible by IL-3, LPS or dexamethasone)
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
round cells growing singly in suspension
Medium:
80-90% mixture of MEM with Earle's salts + Dulbecco's MEM (at 1:1) + 10-20% h.i. FBS + 2x non-essential amino acids + 2x vitamins
Subculture:
maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 3-5 days; seed out at 0.5-0.7 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-50 hours
Harvest:
saturation density at ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species:
originally confirmed as murine with IEF of MDH, NP and recently as mus musculus by COI DNA barcoding
Cytogenetics:
murine hypodiploid karyotype with 20% polyploidy - 30(29-30)<2n>XY, 6-8 centric fusion markers present
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -

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