MB49 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

MB49 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Designations: MB49
Biosafety
Level:
1
Medium &
Serum:
See Propagation
Organism: Mus musculus (mouse)
Source: Organ: Bladder
Disease: adenocarcinoma
Related Cell Culture Products
Morphology: epithelial
Growth
Properties:
adherent
Propagation: The base medium for this cell line is formulated RPMI-1640 Medium. To make the complete
growth medium, add the following components to the base medium: fetal bovine serum to
a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered
saline (D-PBS) or 0.25%(w/v) Trypsin - 0.53 mM EDTA solution to remove all traces
of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an
inverted microscope until cell layer is dispersed (usually within 5 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while
waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C
to facilitate dispersal.
4. Add 6.0 to 8.0ml of complete growthmedium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum
of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.
6. Incubate cultures at 37C. We recommend that you subculture when the culture
reaches a cell concentration between 8 X 10(4) and 1.5 X 10(5) cells/sq. cm.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.
Medium renewal: Every 2 to 3 days
Preservation: Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5%
Storage temperature liquid nitrogen vapor phase
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