pAC-85b BioVector NTCC质粒载体菌种细胞基因保藏中心

pAC-85b BioVector NTCC质粒载体菌种细胞基因保藏中心

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pAC85b Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase
Use to confirm the function of the products of genes or cDNAs that are thought to encode a phytoene synthase enzyme, or to screen genomic or cDNA libraries for plasmids containing such genes (crtB) or cDNAs (psy). The plasmid pAdPSY1 can be used as a positive control. Grow in liquid culture on a platform shaker at 28 degrees Celsius in darkness for 2-3 days, or on agar plates at room temperature for 3-7 days. A yellow colony color and an accumulation of beta-carotene is indicative of phytoene synthase activity.

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