NTL4(pZLR4) strain农杆菌菌株群体感应检测报告菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NTL4(pZLR4) strain农杆菌菌株群体感应检测报告菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Deposited As Agrobacterium tumefaciens
Strain Designations NTL4(pZLR4)
Application Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals.
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
Biosafety Level 1
Type Strain no
Comments The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention.
Pzlr4 confers resistance to beta lactam antibiotics and gentamicin.
The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity.
Medium LB medium with 15 mcg/ml gentamycin
Growth Conditions Temperature: 28°C
Atmosphere: Aerobic
Isolation This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
References Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399

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