pETcoco-1拷贝数可调节型原核表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pETcoco-1拷贝数可调节型原核表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pETcoco-1拷贝数可调节型原核表达载体，阿拉伯糖诱导表达trfA基因使质粒拷贝数由单拷贝增加值中等拷贝，降低本底表达提高重组质粒稳定性。T7 lac启动子，His和S标签，EK蛋白酶切割位点，HSV标签，Cm氯霉素抗性。
The pETcoco™ expression vectors are designed to allow “on command” amplification of vector copy number
from single copy (1–2 plasmid copies per cell) to medium copy (20– 50 per cell). The vector is maintained in the
single copy state by utilizing the oriS and repE elements of the F episome together with the parABC partition
determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV.
The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with
arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and
extremely low basal expression levels in the single copy state, while allowing robust target protein expression
typical of the pET vectors. Unique restriction sites are shown on the circle map (below). The sequence is
numbered relative to the start of T7 transcription at +1.
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net