pFastBac HT A BioVector NTCC质粒载体菌种细胞基因保藏中心

pFastBac HT A BioVector NTCC质粒载体菌种细胞基因保藏中心
pFastBacHTA载体基本信息
出品公司: Invitrogen
载体名称: pFastBacHTA, pFastBac HTA, pFastBacHT A, pFastBac HT A
质粒类型: 昆虫细胞表达载体；杆状病毒表达载体
启动子: Polyhedrin
表达水平: 高
克隆方法: 多克隆位点，限制性内切酶
载体大小: 4856 bp
5' 测序引物: pFastbac-F: TATTCCGGATTATTCATACC
3' 测序引物: pFastBac-R: ACAAATGTGGTATGGCTGA
载体标签: N-His tag
载体抗性: Ampicillin 氨苄
筛选标记: Gentamicin 庆大霉素
备注:
含TEV切割位点
产品目录号: 10584-027
稳定性: 稳表达 Stable
组成型: 组成型 Constitutive
病毒/非病毒: 杆状病毒
pFastBacHTA载体质粒图谱和多克隆位点信息
pFastBacHT A 载体图谱



pFastBacHT A 多克隆位点

pFastBacHT A 载体特征
pFastBacHTA载体简介
Bac-to-BacHT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后，在 Sf9、Sf21 或 High Five 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBacHT 载体具

有以下特点：

用于表达蛋白质的多角体强启动子

用于简化克隆的三个读码框

用于轻松纯化重组融合蛋白的 N-端 6xHis 标签

用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点



引用及参考文献

DEF6, a Novel Substrate for the Tec Kinase ITK, Contains a Glutamine-rich Aggregation-prone Region and Forms Cytoplasmic Granules that Co-localize with P-bodies.
Hey F, Czyzewicz N, Jones P, Sablitzky F,
J Biol Chem (2012) 287:31073-31084
Product usage: na

Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Dan C; Kelly A; Bernard O; Minden A;
J Biol Chem (2001) 276:32115-32136
Product usage: pFASTBAC/HT and pFASTBAC-1 vectors were used to transfect with Sf9 cells with Cellfectin.

Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Kennett Sarah B; Moorefield K Scott; Horowitz Jonathan M;
J Biol Chem (2002) 277:9780-9789
Product usage: Baculovirus stocks encoding Sp1, Sp3, M1, and M2 were prepared using the PCR, appropriate primers, and pCMV4-Sp1/flu, pBSK-Sp3/flu, pCR-M1/flu,

and pCR-M2/flu as substrates. PCR reactions employed pGEX-M2 using Taq polymerase. Amplified cDNAs were subcloned in pFASTBacHTA.
pFastBacHTA载体序列
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net