pD2610-v1，pD2610-v1-03 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pD2610-v1，pD2610-v1-03 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Mammalian Transient Expression Vectors
These vectors are primarily designed for high level protein expression in transiently transfected cells.
Mammalian Transient Expression Vectors: Vector GPS
Vector GPS was used to develop a panel of transient expression vectors containing various combinations of enhancers, promoters, introns,
polyadenylation sequences and viral plasmid amplification systems. Custom combinations are also available. These vectors do not contain
mammalian selectable markers to minimize size and maximize transfection efficiency.
Name Qty Storage
pD2610-v1-03 2µg -20℃
Standard Transfection Protocol
1. 24 hours before transfection seed cells (in antibiotic free media) to be 70-90% Confluent at time of transfection. (6 well plate: 0.25-1.0x106
,
24 well plate: 0.5-2.0x105
, 96 well plate: 1-4x104
)
2. *Dilute Lipofectamine in DMEM or Opti-MEM (LifeTech)
3. *Dilute DNA (endotoxin free is optimal) in DMEM or Opti-MEM (LifeTech)
4. Add diluted DNA mixture to diluted Lipofectamine mixture.
5. Incubate for 10 minutes.
6. Add DNA-Lipid complex to cells
7. Visualize cells using microscope and analyze.

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