pGL4.13[luc2/SV40]哺乳动物细胞荧光素酶质粒

pGL4.13[luc2/SV40]哺乳动物细胞荧光素酶质粒
pGL4.13[luc2/SV40]哺乳动物细胞荧光素酶报告质粒载体，Luc2荧光报告基因，SV40 early增强子-启动子，Amp抗性
启动子: SV40 复制子: pUC ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳细胞，信号通路报告载体 质粒大小: 4641bp 原核抗性: 氨苄青霉素Amp 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃，有氧 LB 表达宿主: 哺乳细胞 诱导方式: 无须诱导，瞬时表达 5'测序引物: RVP3:CTAGCAAAATAGGCTGTCCC 3'测序引物: 根据序列设计引物 质粒简介 The pGL4 Luciferase Reporter Vectors are the next generation of reporter gene vectors optimized for expression in mammalian cells. Numerous configurations of pGL4 Vectors are available, including those with the synthetic luc2 (Photinus pyralis) and hRluc (Renilla reniformis) genes, which have been codon optimized for more efficient expression in mammalian cells. Furthermore, both the reporter genes and the vector backbone, including the bla (β-lactamase or Ampr) gene, have been engineered to reduce the number of consensus transcription factor binding sites, reducing background and the risk of anomalous transcription. 质粒图谱
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