pSecTag2 A哺乳动物细胞分泌表达质粒

pSecTag2 A哺乳动物细胞分泌表达质粒
启动子: CMV 复制子: pUC ori，FI ori 终止子: SV40 poly(A) signal,BGH poly(A) signal 质粒大小: 5159bp 原核抗性: 氨苄青霉素Amp 筛选标记: 博来霉素Zeo 克隆菌株: 大肠杆菌DH5α 培养条件: 37℃ 有氧，LB 表达宿主: 哺乳细胞 诱导方式: 无须诱导，瞬时表达 5'测序引物: T7:TAATACGACTCACTATAGGG 3'测序引物: BGH:TAGAAGGCACAGTCGAGG 质粒简介 pSecTag2 A, B, and C vectors are fusion vectors requiring that you clone your gene of interest in frame with the initiation ATG of the N-terminal Igκ-chain leader sequence and/or the C-terminal myc epitope/polyhistidine tag. Three versions of this vector are provided to facilitate cloning. For proper expression,first determine which restriction sites are appropriate for ligation and then which vector will preserve the reading frame at BOTH the 5′ and the 3′ ends. It may be nece ssary to PCR your gene product to create a fragment with the appropriate restriction sites to clone in frame at both ends. Carefully inspect your gene and the multiple cloning site of each vector before cloning your gene of interest. 质粒图谱
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