pRevTRE哺乳动物细胞调控质粒

pRevTRE哺乳动物细胞调控质粒
启动子: CMV 复制子: pUC ori 质粒分类: 哺乳细胞，四环素调控系统载体 质粒大小: 6487bp 原核抗性: Amp 筛选标记: Hyg 克隆菌株: Stbl3 培养条件: 37℃，有氧 LB 表达宿主: 哺乳细胞 诱导方式: 四环素诱导 5'测序引物: 根据序列设计引物 3'测序引物: 根据序列设计引物 质粒简介 pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tetresponse element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described (1). The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Ψ+ (the extended viral packaging signal) and the hygromycin resistance (Hygr) gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously (2, 3). pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria. 质粒图谱
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