Super PiggyBac Transposase (PB200PA-1)哺乳动物细胞转座质粒

Super PiggyBac Transposase (PB200PA-1)哺乳动物细胞转座质粒
启动子: Polr2A promoter 复制子: pUC ori,f1 ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳细胞转座酶表达质粒 质粒大小: 8531bp 原核抗性: 氨苄青霉素Amp 克隆菌株: 大肠杆菌Stbl3 培养条件: 37℃，有氧，LB 表达宿主: 哺乳细胞 诱导方式: 无须诱导，瞬时表达 5'测序引物: M13R:CAGGAAACAGCTATGACC 3'测序引物: 根据序列设计引物 质粒简介 Super PiggyBac Transposase (PB200PA-1)是一个哺乳细胞转座质粒。The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes.The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells. 质粒图谱
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