LLC-GFP-luc小鼠肺癌细胞cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

LLC-GFP-luc小鼠肺癌细胞cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Luc-GFP-LLC cell line
Cat No.: NTCC910343

General Information:
Organism: Homo sapiens, human
Tissue: embryo
Culture Properties: adherent, Luciferase and GFP stably expression.
Morphology: fibroblast
Culture Method:
Complete Growth Medium: DMEM (Gibco 12800-017)+10% FBS+ Penicillin/Streptomycin
Volumes are given for a 25cm2 flask. Increase or decrease the
amount of dissociation medium needed proportionally for culture
vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA
solution to remove all traces of serum that contains trypsin
inhibitor.
Subculturing: 3. Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and
observe cells under an inverted microscope until cell layer is
dispersed. Discard Trypsin-EDTA solution.
4. Add 6.0 to 8.0mL of complete growth medium and aspirate
cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture
vessels.
6. Incubate cultures at 37°C, 5% CO2.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Freeze medium: Complete growth medium supplemented
with
Cryopreservation: 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net