C58C1 strain农杆菌菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

C58C1 strain农杆菌菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
Description

Ti plasmid-less and Rifr derivative of C58; Rifr


Storage of Agrobacterium Strains by Low-Temperature Freezing

1. Inoculate 2 or 3 mL of YEB media that contains the appropriate antibiotics for plasmid maintenance with your strain).
2. Grow the culture to early or middle stationary phase (usually overnight). Chill the culture and the sterile medium containing 50% glycerol on ice.
3. Add an equal volume of the medium with glycerol to make the culture 25% glycerol. Mix culture and medium completely.
4. Transfer the cells to a labeled 2-mL tube, and place it in the freezer. The frozen culture can be stored indefinitely.
5. To recover the strain, simply scrape a bit of the frozen culture onto plate medium
containing the appropriate antibiotics. This can be done with a sterile, cooled,
inoculating loop or with a sterile pipet tip. It is important to handle the culture
quickly to prevent thawing and refreezing. If handled properly, the same culture
can used repeatedly for retrieving the bacteria strain.

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net