pMOD4 galK-GT plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMOD4 galK-GT plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Vector backbone pMOD4
(Search Vector Database)
Backbone manufacturer Epicentre
Backbone size w/o insert (bp) 2100
Vector type Recombineering
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) EC100pir116
Growth instructions EC100pir116 or other pir containing bacteria
Copy number Low Copy
GENE/INSERT
Gene/Insert name galK
Species E.coli
Insert Size (bp) 1612
Entrez Gene galK (a.k.a. b0757, ECK0746, JW0740, galA)
Tags / Fusion Proteins
50 nucleotides from N-term TAP, C-term TAP, and FLAG-GFP (N terminal on insert)
50 nucleotides from N-term TAP, C-term TAP, and FLAG-GFP (C terminal on insert)
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site SphI (not destroyed)
3′ cloning site SacI (not destroyed)
5′ sequencing primer GTCAGTGAGCGAGGAAGCGGAAG
3′ sequencing primer ATTCAGGCTGCGCAACTGT
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net