pUCP19广宿主绿脓杆菌穿梭表达载体Pseudomonas aeruginosa质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pUCP19广宿主绿脓杆菌穿梭表达载体Pseudomonas aeruginosa质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pUCP19 in Escherichia coli DH5alphaF’
Depositors HP Schweizer, University of Calgary
Biosafety Level 1
Host Host range:
Broad host range; Pseudomonas aeruginosa; Escherichia coli

Host requirements:
E. coli lacIq
E. coli lacZdeltaM15 for recombinant screening
P. aeruginosa lacIq
P. aeruginosa lacZdeltaM15 for recombinant screening

Suggested hosts:
Pseudomonas aeruginosa PAO1-LAC (ATCC 47085)
Pseudomonas aeruginosa PA103-LAC (ATCC 47086)
Vector Information
Size (kb): 4.5
Vector: pUCP19 (plasmid)
Promoters: Promoter for expression lac
Construction: pUC19, pRO1614
Marker(s): ampR,CbR
Construct size (kb): 4.5
Features: marker(s): ampR, CbR
promoter for expression: lac
replicon: bhr (rep pRO1600)
replicon: pMB1
MCS: EcoRI...HindIII
coding sequence: lacZalpha
Applications
Expression vector
Shuttle vector
Vector containing primer sites useful for sequencing
Vector permitting construction of fusion proteins
Vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.5; EcoRI--4.5; HindIII--4.5; EcoRI/PvuII--3.7, 0.47, 0.31, 0.16; HincII--3.9, 0.6; PvuI--2.7, 1.8.

Plasmid is maintained in P. aeruginosa at 10-25 copies per cell.

Vector allows screening of recombinants on LB XGal indicator medium as well as on MacConkey lactose medium.

Shuttle expression vector allowing visual detection of recombinant molecules in a broad host range.

Constructed by insertion of a 1.8 kb PstI fragment from pRO1614 into the NarI site of pUC19.

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net