IVA12 cell line小鼠细胞株细胞系HB-145 BioVector NTCC质粒载体菌种细胞基因保藏中心

IVA12 cell line小鼠细胞株细胞系HB-145 BioVector NTCC质粒载体菌种细胞基因保藏中心

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against HLA DR, DP, DQ
Cellular Products
immunoglobulin; monoclonal antibody; against HLA DR, DP, DQ
Comments
Animals were immunized with the Priess human B cell line.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 105 cells/ml and maintain between 1 X 105 and 1 X 106 cells/ml. Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1.0 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 cells/mL.
Isotype IgG1; kappa light chain
Antigenic Determinant HLA DR, DP, DQ
【Supplier来源】BioVector NTCC Inc.
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【Website网址】 http://www.biovector.net