pPIC9酵母组成型分泌表达质粒载体图谱序列抗性说明书-BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥4920
- 货 号：Plasmid pPIC9
- 产 地：北京
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- 联系人：Dr.Xu, Biovector NTCC Inc.
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载体基本信息
| Invitrogen |
| |
| pPIC9, pPIC 9 |
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| 毕赤酵母蛋白表达载体 |
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| 高拷贝 |
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| AOX1 |
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| 多克隆位点，限制性内切酶 |
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| 8023 bp |
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| 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´； alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
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| 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´ |
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| / |
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| Ampicillin |
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| HIS4 |
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利用alpha分泌因子，分泌表达蛋白。 |
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| K1710-01 |
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| 稳定 Stable |
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| 组成型 Constitutive |
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| 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
pPIC9载体的详细信息如下：
• pPIC9载体大小8,023 bp，是融合表达载体。
• 载体构建过程中，可供使用的单一限制性内切酶位点为Xho I, SnaB I, EcoR I, Avr II, Not I.
• 利用alpha因子分泌信号肽，分泌表达蛋白基因。
• 在载体构建过程中，你的基因必须保证与信号肽的起始密码子的读码框一致。
• 毕赤酵母中利用HIS4进行筛选。
• 为了将基因插入GS115或KM71的AOX区, 使用SacI限制性内切酶线性化质粒(GS115中产生His+Mut+基因型，KM71中产生His+ MutS基因型)
•为了将基因插入GS115或KM71的His4区, 使用Sal I或者Stu I限制性内切酶线性化质粒(GS115中产生His+ Mut+基因型，KM71中产生His+ MutS基因型)
•将基因插入GS115的AOX1区域，需要使用Bgl II线性化质粒 (产生His+MutS基因型)。
载体序列
LOCUS pPIC9 8023 bp ds-DNA circular SYN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
1201 agagaggctg aagcttacgt agaattccct agggcggccg cgaattaatt cgccttagac
1261 atgactgttc ctcagttcaa gttgggcact tacgagaaga ccggtcttgc tagattctaa
1321 tcaagaggat gtcagaatgc catttgcctg agagatgcag gcttcatttt tgatactttt
1381 ttatttgtaa cctatatagt ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc
1441 ttgctcctga tcagcctatc tcgcagctga tgaatatctt gtggtagggg tttgggaaaa
1501 tcattcgagt ttgatgtttt tcttggtatt tcccactcct cttcagagta cagaagatta
1561 agtgagaagt tcgtttgtgc aagcttatcg ataagcttta atgcggtagt ttatcacagt
1621 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc
1681 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
1741 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
1801 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
1861 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
1921 cgaccacacc cgtcctgtgg atctatcgaa tctaaatgta agttaaaatc tctaaataat
1981 taaataagtc ccagtttctc catacgaacc ttaacagcat tgcggtgagc atctagacct
2041 tcaacagcag ccagatccat cactgcttgg ccaatatgtt tcagtccctc aggagttacg
2101 tcttgtgaag tgatgaactt ctggaaggtt gcagtgttaa ctccgctgta ttgacgggca
2161 tatccgtacg ttggcaaagt gtggttggta ccggaggagt aatctccaca actctctgga
2221 gagtaggcac caacaaacac agatccagcg tgttgtactt gatcaacata agaagaagca
2281 ttctcgattt gcaggatcaa gtgttcagga gcgtactgat tggacatttc caaagcctgc
2341 tcgtaggttg caaccgatag ggttgtagag tgtgcaatac acttgcgtac aatttcaacc
2401 cttggcaact gcacagcttg gttgtgaaca gcatcttcaa ttctggcaag ctccttgtct
2461 gtcatatcga cagccaacag aatcacctgg gaatcaatac catgttcagc ttgagacaga
2521 aggtctgagg caacgaaatc tggatcagcg tatttatcag caataactag aacttcagaa
2581 ggcccagcag gcatgtcaat actacacagg gctgatgtgt cattttgaac catcatcttg
2641 gcagcagtaa cgaactggtt tcctggacca aatattttgt cacacttagg aacagtttct
2701 gttccgtaag ccatagcagc tactgcctgg gcgcctcctg ctagcacgat acacttagca
2761 ccaaccttgt gggcaacgta gatgacttct ggggtaaggg taccatcctt cttaggtgga
2821 gatgcaaaaa caatttcttt gcaaccagca actttggcag gaacacccag catcagggaa
2881 gtggaaggca gaattgcggt tccaccagga atatagaggc caactttctc aataggtctt
2941 gcaaaacgag agcagactac accagggcaa gtctcaactt gcaacgtctc cgttagttga
3001 gcttcatgga atttcctgac gttatctata gagagatcaa tggctctctt aacgttatct
3061 ggcaattgca taagttcctc tgggaaagga gcttctaaca caggtgtctt caaagcgact
3121 ccatcaaact tggcagttag ttctaaaagg gctttgtcac cattttgacg aacattgtcg
3181 acaattggtt tgactaattc cataatctgt tccgttttct ggataggacg acgaagggca
3241 tcttcaattt cttgtgagga ggccttagaa acgtcaattt tgcacaattc aatacgacct
3301 tcagaaggga cttctttagg tttggattct tctttaggtt gttccttggt gtatcctggc
3361 ttggcatctc ctttccttct agtgaccttt agggacttca tatccaggtt tctctccacc
3421 tcgtccaacg tcacaccgta cttggcacat ctaactaatg caaaataaaa taagtcagca
3481 cattcccagg ctatatcttc cttggattta gcttctgcaa gttcatcagc ttcctcccta
3541 attttagcgt tcaacaaaac ttcgtcgtca aataaccgtt tggtataaga accttctgga
3601 gcattgctct tacgatccca caaggtggct tccatggctc taagaccctt tgattggcca
3661 aaacaggaag tgcgttccaa gtgacagaaa ccaacacctg tttgttcaac cacaaatttc
3721 aagcagtctc catcacaatc caattcgata cccagcaact tttgagttgc tccagatgta
3781 gcacctttat accacaaacc gtgacgacga gattggtaga ctccagtttg tgtccttata
3841 gcctccggaa tagacttttt ggacgagtac accaggccca acgagtaatt agaagagtca
3901 gccaccaaag tagtgaatag accatcgggg cggtcagtag tcaaagacgc caacaaaatt
3961 tcactgacag ggaacttttt gacatcttca gaaagttcgt attcagtagt caattgccga
4021 gcatcaataa tggggattat accagaagca acagtggaag tcacatctac caactttgcg
4081 gtctcagaaa aagcataaac agttctacta ccgccattag tgaaactttt caaatcgccc
4141 agtggagaag aaaaaggcac agcgatacta gcattagcgg gcaaggatgc aactttatca<br style="PADDING-BOTTOM: 0px; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RI
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
1201 agagaggctg aagcttacgt agaattccct agggcggccg cgaattaatt cgccttagac
1261 atgactgttc ctcagttcaa gttgggcact tacgagaaga ccggtcttgc tagattctaa
1321 tcaagaggat gtcagaatgc catttgcctg agagatgcag gcttcatttt tgatactttt
1381 ttatttgtaa cctatatagt ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc
1441 ttgctcctga tcagcctatc tcgcagctga tgaatatctt gtggtagggg tttgggaaaa
1501 tcattcgagt ttgatgtttt tcttggtatt tcccactcct cttcagagta cagaagatta
1561 agtgagaagt tcgtttgtgc aagcttatcg ataagcttta atgcggtagt ttatcacagt
1621 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc
1681 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
1741 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
1801 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
1861 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
1921 cgaccacacc cgtcctgtgg atctatcgaa tctaaatgta agttaaaatc tctaaataat
1981 taaataagtc ccagtttctc catacgaacc ttaacagcat tgcggtgagc atctagacct
2041 tcaacagcag ccagatccat cactgcttgg ccaatatgtt tcagtccctc aggagttacg
2101 tcttgtgaag tgatgaactt ctggaaggtt gcagtgttaa ctccgctgta ttgacgggca
2161 tatccgtacg ttggcaaagt gtggttggta ccggaggagt aatctccaca actctctgga
2221 gagtaggcac caacaaacac agatccagcg tgttgtactt gatcaacata agaagaagca
2281 ttctcgattt gcaggatcaa gtgttcagga gcgtactgat tggacatttc caaagcctgc
2341 tcgtaggttg caaccgatag ggttgtagag tgtgcaatac acttgcgtac aatttcaacc
2401 cttggcaact gcacagcttg gttgtgaaca gcatcttcaa ttctggcaag ctccttgtct
2461 gtcatatcga cagccaacag aatcacctgg gaatcaatac catgttcagc ttgagacaga
2521 aggtctgagg caacgaaatc tggatcagcg tatttatcag caataactag aacttcagaa
2581 ggcccagcag gcatgtcaat actacacagg gctgatgtgt cattttgaac catcatcttg
2641 gcagcagtaa cgaactggtt tcctggacca aatattttgt cacacttagg aacagtttct
2701 gttccgtaag ccatagcagc tactgcctgg gcgcctcctg ctagcacgat acacttagca
2761 ccaaccttgt gggcaacgta gatgacttct ggggtaaggg taccatcctt cttaggtgga
2821 gatgcaaaaa caatttcttt gcaaccagca actttggcag gaacacccag catcagggaa
2881 gtggaaggca gaattgcggt tccaccagga atatagaggc caactttctc aataggtctt
2941 gcaaaacgag agcagactac accagggcaa gtctcaactt gcaacgtctc cgttagttga
3001 gcttcatgga atttcctgac gttatctata gagagatcaa tggctctctt aacgttatct
3061 ggcaattgca taagttcctc tgggaaagga gcttctaaca caggtgtctt caaagcgact
3121 ccatcaaact tggcagttag ttctaaaagg gctttgtcac cattttgacg aacattgtcg
3181 acaattggtt tgactaattc cataatctgt tccgttttct ggataggacg acgaagggca
3241 tcttcaattt cttgtgagga ggccttagaa acgtcaattt tgcacaattc aatacgacct
3301 tcagaaggga cttctttagg tttggattct tctttaggtt gttccttggt gtatcctggc
3361 ttggcatctc ctttccttct agtgaccttt agggacttca tatccaggtt tctctccacc
3421 tcgtccaacg tcacaccgta cttggcacat ctaactaatg caaaataaaa taagtcagca
3481 cattcccagg ctatatcttc cttggattta gcttctgcaa gttcatcagc ttcctcccta
3541 attttagcgt tcaacaaaac ttcgtcgtca aataaccgtt tggtataaga accttctgga
3601 gcattgctct tacgatccca caaggtggct tccatggctc taagaccctt tgattggcca
3661 aaacaggaag tgcgttccaa gtgacagaaa ccaacacctg tttgttcaac cacaaatttc
3721 aagcagtctc catcacaatc caattcgata cccagcaact tttgagttgc tccagatgta
3781 gcacctttat accacaaacc gtgacgacga gattggtaga ctccagtttg tgtccttata
3841 gcctccggaa tagacttttt ggacgagtac accaggccca acgagtaatt agaagagtca
3901 gccaccaaag tagtgaatag accatcgggg cggtcagtag tcaaagacgc caacaaaatt
3961 tcactgacag ggaacttttt gacatcttca gaaagttcgt attcagtagt caattgccga
4021 gcatcaataa tggggattat accagaagca acagtggaag tcacatctac caactttgcg
4081 gtctcagaaa aagcataaac agttctacta ccgccattag tgaaactttt caaatcgccc
4141 agtggagaag aaaaaggcac agcgatacta gcattagcgg gcaaggatgc aactttatca<br style="PADDING-BOTTOM: 0px; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RI
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