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pGMAAV-CMV-MCS-3flag-EF1-ZsGreen腺相关病毒载体AAV质粒-BioVector NTCC保藏中心

  • 价  格:¥39865
  • 货  号:pGMAAV-CMV-MCS-3flag-EF1-ZsGreen
  • 产  地:北京
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pGMAAV-CMV-MCS-3flag-EF1-ZsGreen腺相关病毒载体AAV质粒-BioVector NTCC保藏中心
Expression表达类型:overexpression
Promoter启动子:CMV
Reporter报告基因:ZsGreen
Cre-dependent重组酶依赖性:No
Tag标签:3FLAG(C-terminal)
Cat. No.:P-AAVV-B03
Description描述: AAV Vector System, also named AAV expression system or AAV packaging plasmid system, is powerful tool for in-vivo gene delivery, gene editing and gene therapy. You can easily produce recombinant AAV (rAAV) paticle in 293T cell line in high titer using AAV Vector System. The Genemedi AAV vector system including multiple AAV expression vector plasmids, AAV helper plasmid and the serotypes-specific AAV Rep-Cap plamids. AAV expression vectors have been inserted with differernt expression cassettes, containing kinds of verified protomters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The AAV expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA AAV expression vector. The serotypes-specific AAV Rep-Cap plamid (AAV-RC plasmid, or called AAV-RC plasmid) contain the AAV2-Rep gene with different serotypes of AAV's Cap gene(also called AAV capsids gene). AAV Rep-Cap plasmids is including AAV2, AAV5, AAV6, AAV8, AAV9, AAV-PHP.B, AAV-PHP.eB, AAV PHP.s, AAV-Retro (Retrograde), AAV-Anc80 (L65), AAV-DJ, AAV-DJ8. GeneMedi also supplies capsid optimized AAV variant including AAV2 variant(Y444F), AAV2 variant (Y272F, Y444F, Y500F, Y730F), AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F), AAV2.7m8, AAV8 variant (Y733F), AAV8 variant(Y733F, Y447F), AAV8 variant(Y733F,Y447F,Y275F) and some other engineering AAV serotypes not mentioned.
Advantages 1. Safety. The wild type Adeno Associated Virus (AAV) has not currently been known to cause disease, and further security of recombinant AAV is ensured after removal of most AAV genome elements. 2. Low immunogenicity. AAV causes a very mild immune response, lending further support to its apparent lack of pathogenicity. 3. Broad range of host and specificity targeting. AAV has the ability to infect both dividing and quiescent cells, allowing genetic material to be delivered to a highly diverse range of cell types. More than 12 AAV serotypes and a variety of capsid engineered AAV vectors can be selected according to their tissue tropisms. 4. Long-term stable expression. Long term and stable expression of genes in vivo can be mediated by AAV. 5. Stable physical properties. AAV is still alive at 60℃ and resistant to chloroform.
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