Detroit 562 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

Detroit 562 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human
Tissue pharynx: derived from metastatic site: pleural effusion
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease pharyngeal carcinoma
Age adult
Gender female
Ethnicity Caucasian
Karyotype Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock. Cytogenetic instability has been reported in the literature for some cell lines
Clinical Data adult
Caucasian
female
Genes Expressed keratin
Cellular Products keratin
Virus Susceptibility Human poliovirus 1
Vesicular stomatitis virus
Comments The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
TH01: 8,9
TPOX: 8,10
vWA: 16
Isoenzymes G6PD, B
References Peterson WD Jr., et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. PubMed: 5668122
Peterson WD Jr., et al. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971. PubMed: 5554463

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