KMS-12-BM cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

KMS-12-BM cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
KMS-12-BM
Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from the bone marrow of a 64-year-old woman with multiple myeloma in 1988; sister cell line of KMS-12-PE (DSM ACC 606). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
mostly single cells in suspension, 1-2% giant cells;
image:


Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/m; split saturated culture 1:2 every 2-3 days; maintain at ca. 0.5-1.0 x 106 cells/ml; first week after thawing cultivate in 24-well-plate, initially cells grow slowly
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays

Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image

Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile (identical to that of sister cell line KMS-12-PE; ACC 606)

Species:
confirmed as human by cytogenetics

Cytogenetics:
human flat-moded hypertriploid karyotype with 3% polyploidy - 69-79<3n>XX, -X, +1, +1, -4, +5, +6, +8, +9, -10, +13, +14, -15, -16, +17, -18, +19, +20, +21, -22, +4-5mar, der(1)t(1;4)(q11;q32), del(1)(p21)x2, der(1;8)(q10;q10)x2, add(4)(q32)x1-2, der(5;17)(p10;q10)x2, add(6)(q2?5), der(9)add(9)(p24)t(9;11)(q34;q13), der(9)t(9;11)(q34;q13), der(11)t(11;14)(q13;q32)x2-3, idic(?;13)(?;q32-33)x2, der(14)t(11;14)(q13;q32), der(17)t(11;17)(q21.2;q22)t(11;14)(q13;q32)x2, add(19)(q13), add(19)(q13) - related to published karyotype - carries semi-cryptic t(11;14) with IGH-CCND1 rearrangement

Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net